Claudins by Alan S. L. Yu (Eds.)

By Alan S. L. Yu (Eds.)

This quantity of present issues in Membranes makes a speciality of Membrane Protein Crystallization, starting with a assessment of prior successes and common traits, then additional discussing demanding situations of mebranes protein crystallization, cellphone loose construction of membrane proteins and novel lipids for membrane protein crystallization. This ebook additionally comprises instruments to enchance membrane protein crystallization, procedure developments, and crystallization concepts used for photosystem I and its complexes, developing Membrane Protein Crystallization as a wanted, sensible reference for researchers.

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Introduction: Claudins, Tight Junctions, and the Paracellular Barrier 9 TABLE I Predicted Functions of Claudin Subtypes to Ion Permeation Evaluated in Overexpression or Knockdown in Cultured Epithelial Cells Claudin Predicted function Reference Clandin-1 Ion barrier Inai, Kobayashi, and Shibata (1999), McCarthy et al. (1996) Claudin-2 Cation channel Amasheh et al. (2002), Hou, Gomes, Paul, and Goodenough (2006), Van Itallie et al. (2003), Yu et al. (2009) Claudin-4 Cation barrier Van Itallie, Rahner, and Anderson (2001) Cation barrier or anion channel Hou et al.

Tsukita, S. (1999). Manner of interaction of heterogeneous claudin species within and between tight junction strands. Journal of Cell Biology, 147, 891–903. , Southwood, C. , et al. (2004). Deafness in Claudin 11-null mice reveals the critical contribution of basal cell tight junctions to stria vascularis function. The Journal of Neuroscience, 24, 7051–7062. , Southwood, C. , Li, J. , Riordan, G. , Brodie, S. , et al. (1999). CNS myelin and sertoli cell tight junction strands are absent in Osp/claudin-11 null mice.

2000; A B C FIGURE 2 Confocal microscopy of MDCK II cell monolayers. Monolayers are labeled by immunofluorescence for (A) claudin-7 (CY3, red) and (B) occludin (Alexa 488, green). (C) Both of these tight junction proteins colocalize at the tight junction (yellow). In the XZ view of the tight junction, claudin-1 appears to extend slightly more basally than occludin. Scale bar equals 10 m. 27 2. Morphology of Claudins Van Itallie, Rahner, & Anderson, 2001; Yu, Enck, Lencer, & Schneeberger, 2003).

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