Bacterial Wilt Disease: Molecular and Ecological Aspects by A. Kelman (auth.), Dr. Philippe Prior, Dr. Caitilyn Allen

By A. Kelman (auth.), Dr. Philippe Prior, Dr. Caitilyn Allen Ass. Prof., Dr. John Elphinstone (eds.)

Jointly released with INRA, Paris.

Bacterial wilt, as a result of Ralstonia solanacearum, is a truly harmful plant disorder that assaults over 450 diversified species, together with the various most vital monetary crop vegetation. usually endemic, the bacterium transmits during the soil, penetrates the plant root method and at last factors irreversible wilting and loss of life. This e-book summarizes the present details on bacterial wilt for either the elemental examine neighborhood and for involved pros who're confronted with the affliction within the box, providing the newest methods to prognosis and keep watch over of the disorder. Emphasis is put on built-in and biologically sustainable keep watch over equipment. additionally provided is the latest genetic/biochemical study exploring the interplay among the bacterium and its plant host on the molecular level.

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1996) found that the strains of R. solanacearum sequenced fell into the two divisions Phylogeny and Diagnostics of R. solanacearum 29 described by Cook et al. (1989) based upon RFLP data. Taghavi et al. (1996) also showed that the two closely related organisms P. syzygii and the BDB clustered within the R. solanacearum species complex. These organisms formed a further subdivision, with certain R. solanacearum isolates originating from Indonesia, closely related to division 2 (Taghavi et al. 1996).

Each reaction was overlaid with mineral oil and subjected to 25 cycles of 92°oC for 1 min, 62°oC for 1 min and 72°oC for 2 min after an initial denaturation at 92°oC for 3 min. Amplification products were completed by a final extension period of 72°oC far 10min. 16S-23S rRNA Gene Intergenie Spacer Region. PCR amplifications were routinely perforrned on an MJ Research PTC 100 thermocycler. Reaction Phylogeny and Diagnostics of R. 25 mM of each primer (ITSallF, ITSDIVIF, ITSDIV2F, PsALLR) and 25 ng of purified DNA.

1993; Gillis et al. 1995). R. solanacearum is a heterogeneous species showing significant phenotypic diversity (Cook et al. 1989; Hayward 1991). Traditionally R. solanacearum strains have been divided into five races based on host range and five biovars based on biochemical properties (Hayward 1964 1991). Two clusters within strains of R. solanacearum have been reported based on restriction fragment length polymorphism (RFLP) (Cook et al. 1989, 1991) and 16S rDNA sequences (Li et al. 1993; Taghavi et al.

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