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This tradition from the Upadesha sequence of Dzogchen permits the practitioner to acknowledge the country of the dream and to exploit it for perform hence constructing readability of the kingdom of contemplation. Teachings given at Subiaco (Italy) in July 1976.
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Extra info for Arabidopsis : a laboratory manual
Screening the T l generation has the advantage that any phenotype observed is likely to be dominant, since T l plants are normally hemizygous for a T-DNA insertion (Ye et al. 1999) (see Vacuum Infiltration of Arabidopsis in Chapter 5). The dominant behavior of a mutation should be confirmed in subsequent generations. As with any other insertional mutant, it must be confirmed that the dominant mutation is linked genetically to a T-DNA insertion. Adjacent sequences can then be recovered either by TAIL-PCR (thermal asymmetric interlaced-polymerase chain reaction) (see Chapter 6) or by plasmid rescue.
Trends Plant Sei. 5: 2229. , and Dean C. 1992. Development of an efficient two-element transposon tagging system in Arabidopsis thaliana. Mol. Gen. Genet. 233: 449-461. , and Haseloff J. 1998. Stomata patterning on 38 \: ARABIDOPSIS: A LABORATORY MANUAL the hypocotyl of Arabidopsis thaliana is controlled by genes involved in the control of root epidermis patterning. Dev. Biol. 194: 226-234. R. 1993. Control of flower development in Arabidopsis thaliana by A PETA I Al and interacting genes. Development 119: 721-743.
1 % agarose, and use a Pasteur pipette to sow the seeds on soil (see Chapter 1). If collecting M7 seeds from individual Mj plants, grow the plants at a density of 50-100 per 2-ft2 flat. If collecting M, seeds in pools, grow the plants at a density of 500-1000 per 2-ft2 flat. Collect all of the M, seeds when the plants have reached maturity. 2% EMS exposure for 15 hours generally gives a reasonable level of mutagenesis. However, the efficiency of mutagenesis does vary, so it may be desirable to vary the severity of the treatment by altering the EMS concentration; EMS is unstable over the course of 15 hours, so it is difficult to control the effects of varying times of exposure.