By Niklaus Wirth
One of many vintage texts on information constructions and algorithms! Examples are in Pascal.
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Note several shapes of epithelial cells: columnar cells with well-deﬁned borders in the collecting duct (CD), squamous cells in the thin segment (TS) of the nephron, and even more ﬂattened cells lining blood vessels, the vasa recta in the kidney (VR). ϫ380. b. Dorsal root ganglion cells. Note the large size of these nerve cell bodies and the large, pale (euchromatic) nuclei (N) with distinct nucleoli. Each ganglion cell is surrounded by ﬂattened satellite cells (S). The size of the ganglion cell and the presence of a euchromatic nucleus, prominent nucleolus, and Nissl bodies (rough-surfaced endoplasmic reticulum visible as darker granules within the cytoplasm) reﬂect the extensive synthetic activity required to maintain the exceedingly long processes (axons) of these cells.
Ice crystal formation is prevented by the use of cryoprotectants, rapid freezing, and extremely small tissue samples. The frozen tissue is then placed in a vacuum in the freeze fracture apparatus and struck with a knife edge or razor blade. The scanning-transmission electron microscope (STEM) combines features of the TEM and SEM to allow electronprobe X-ray microanalysis. CHAPTER 1 of immunocytochemical methods to transmission electron microscopy. Similarly, routine EM autoradiography techniques have been reﬁned for use with transmission electron microscopy (see Fig.
Indd 18 Routine preparation of specimens for transmission electron microscopy begins with glutaraldehyde ﬁxation followed by a buﬀer rinse and ﬁxation with osmium tetroxide. Glutaraldehyde, a dialdehyde, preserves protein constituents by cross-linking them; the osmium tetroxide reacts with lipids, particularly phospholipids. The osmium also imparts electron density to cell and tissue structures because it is a heavy metal, thus enhancing subsequent image formation in the TEM. Ideally, tissues should be perfused with buﬀered glutaraldehyde before excision from the animal.